The Lion Breeding Programme creates and maintains breeding centres. It also carries out studies of the behavior of the Asiatic lions and also practices artificial insemination. One such centre has been established in the Sakkarbaug Zoo at the district headquarters of Junagadh, which has successfully bred about 180 lions. 126 pure Asiatic lions have been given to zoos in India and abroad.  The Asian Lion re-introduction project with the chosen site as Kuno National Park in Madhya Pradesh, is getting its lions from the Gir National Park.

Gir has been used by the Gujarat State Forest Department which adopted the Indian Crocodile Conservation Project in 1977 and released close to 1000 Marsh crocodile reared in Gir rearing centre into the Kamaleshwar lake and other reservoirs and small water bodies in and around Gir.